Hi ​@Saranya.S6 ,

An active connection between the CR and the Bot runner is the fundamental principal behind running any Bots. If that connection is broken, the Bot's execution also will get disrupted. 

Thank you for your reply, it’s not about connection, may be I have rephrase the question as always the server will be in the connected state.

Do we need to keep the server in open screen mode, like connecting the server via RDP and keep the screen as is so that GUI interaction won’t get disturbed. 

My doubt is when we close the RDP then it is not working as expected.

Well, I hope you are referring to the Unattended Automation here. If yes, then you don't have to monitor or keep your RDP session active with you the whole time. You just have to set your Device deployment as RDP and enable the Auto-Login for the Bot Runner account for seamless Bot execution. 

May I know what issue you are facing?

Thank you for your response.

The issue is sometimes GUI interaction not working as expected like mouse click and keystrokes.

Put a Desktop Capture action just before this Mouse click/Keystroke action and see if you are getting a Black screen or not. If yes, then follow the instruction mentioned in the below thread.

Bots Failing to Locate Elements Unattended Mode — Black Screenshots Captured | Community

Hope this will help.