Read barcode in a .png file. 

@Pradeep 9499  you can use existing dll or C# code to conver dll and use in Automation anywhere 

https://www.vintasoft.com/docs/vsbarcode-dotnet/Programming-Barcode-Read_Barcodes_From_Image_File.html

@Pradeep 9499 

You have the option to use the package from Botstore

https://botstore.automationanywhere.com/bot/barcode-package

for Python use pyzbar 

https://www.geeksforgeeks.org/how-to-make-a-barcode-reader-in-python/

Check the follow references for using python in A360

https://docs.automationanywhere.com/bundle/enterprise-v2019/page/enterprise-cloud/topics/aae-client/bot-creator/commands/cloud-python-command.html

https://docs.automationanywhere.com/bundle/enterprise-v2019/page/enterprise-cloud/topics/aae-client/bot-creator/using-the-workbench/cloud-use-python-script-to-join-a-list.html

HTH

Regards.